Covaris e220 user manual

Tissue chip protocols, cultured cells chip protocols, msds information. The versatility of the eseries focusedultrasonicator makes it possible to bring the advantages of afa to numerous biological and chemical applications including, dna and chromatin shearing, tissue homogenization, cell lysis, compound. System degas pumps should be run in advance of and during afa treatments, as detailed in instrument user manuals. Consult the manufacturers recommendations for use of other covaris instruments or sample holders to achieve the same target dna fragment size. Cp02 automated cryoprep system user manual cp01 cryoprep manual. Utilizing a spherical acoustic transducer, specifically designed acoustical cuvettes and advanced electronics, afa delivers controlled energy precisely and accurately to sample volumes from.

Each strip contains 8 microtube and allows great flexibility when processing samples in. Optimized shearing protocols are available for fragment sizes from 150 to 5,000 bp. Fragmentation was performed in 96well format according to manufacturer specifications 14. Two parameters were mod ulated to optimize the yields of gdna fragments in the size range of interest. Documents document view page perkin elmer janus liquid. Covaris assumes no liability regarding the use of a protocol for applications in which it is not intended or with use of non covaris certified consumables. Covaris e220 focused ultrasonicator multisample w vwr mm7.

Table 10 shear settings for covaris eseries instrument sonolab software v7 or later. If youre a talented person, that can get things done and can bring value to the company, then covaris is the best place to work for you. Used covaris e220 focused ultrasonicator sample preparer. Covaris provides tools and technologies to improve preanalytical sample preparation, enable novel drug formulations, and manage compounds in the drug discovery process.

The covaris e220 evolution instrument available at ggbc is mostly used for dna shearing application. Related documents document title document number raindance 3kb shearing pr. Please note while using the e220 and e220evolution, the intensifier. The e220 focusedultrasonicator enables multisample batch preparation, capable of processing a wide range of sample types and volumes. Autoplay when autoplay is enabled, a suggested video will automatically play next. Dna shearing with microtubes covaris water conditioning system. Recommended settings are subject to change without notice. Optimization of covaris settings for shearing bacterial.

Instrument and software developed for highperformance sample. The covaris e220 enables highthroughput sample sonication and processes from one to 96 samples in a 96well plate or in individual glass microtubes. Utilizing a dishshaped acoustic transducer and computercontrolled electronics. Company covaris, inc price get quote get quote for this product. Note this protocol has been optimized using a covaris model e220 instrument and the l covaris microtube. This manual contains operation and maintenance instructions for the covaris e and leseries instruments the e220, e220x and le220. Similarly, insufficient degas levels within the bath may result in poor acoustic coupling and thereby shift the mean fragment size. The e220 focusedultrasonicators are versatile instruments engineered for preanalytical sample processing by adaptive focused acoustics.

Set up the covaris s2 or e210 at the appropriate temperature. See the question what are the settings for the s220 and m220 covaris models. The company refers to this process as adaptive focused acoustic energy. The covaris m220 ultrasonicator provides mechanical shearing of dna to generate tight, highly reproducible fragment distributions. It contains background information essential to the proper use and care of this equipment. It is ideal for the production of dna libraries for nextgeneration sequencing. Contact covaris customer support for further assistance. I have been working at covaris fulltime more than 8 years pros. Each strip contains 8 microtube and allows great flexibility when processing samples in varying batch sizes. Covaris covaris e110 focused ultrasonicator covaris e220 focused ultrasonicator multisample w vwr mm7 recirculating chiller. S220 x user manual page 4 warranty when used in accordance with written instruction and under normal operating conditions, the covaris instruments are guaranteed to be free of defects in material and workmanship for one 1 year from the date of original delivery by an authorized representative. Covaris are your leading technical specialists in physical asset management, analytics, maintenance and change management. The e220 may be programed to process from 1 to 96 samples in a single batch. Covaris e210 user manual uncheck hide file extensions for known file types my boss did lose a lot of money by losing the contract, said dogg.

The e220 can be operated as a standalone unit or easily integrated as part of an automated laboratory system. Covaris s220 ultrasonicator user operated holders and tubes are available for 50ul to 2ml sample volumes. Chiller for sseries, eseries and leseries afa ultrasonicators. This instrument is designed to provide researchers an integrated standalone tool to be used in a laboratory environment. Ele220 series user manual page 4 warranty when used in accordance with written instruction and under normal operating conditions, the covaris instruments are guaranteed to be free of defects in material and workmanship for one 1 year from the date of original delivery by an authorized representative. User manual an automated, batch system for high performance sample preparation using adaptive focused acoustic energy. If you are using a covaris model that is not supported, illumina recommends that you use the covaris e220 settings, found in the e220 user manual, as a starting point. This manual includes sections on the operating instructions, maintenance guidelines, and troubleshooting tips. The e220 evolution enables multisample, batch preparation, capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 8 samples in a single batch. Covaris e220 focusedultrasonicator research homogenizer dnashearing cell lysis duration. The covaris instrument works by using acoustic energy wave. The e220 evolution and the covaris process the e220 evolution focusedultrasonicators are based on covaris adaptive focused acoustics afa technology.

Preparation using adaptive focused acoustic afa energy. With over 18 years experience in consultation and a strong background in industry, we can progress your projects and provide solutions. Dna shearing with covaris focusedultrasonicators, dna shearing for the s220 e220, dna shearing with gtube, trushear buffer dna shearing. Covaris e210 focused ultrasonicator wchiller circulator predecessor to e220 10808 duration. This kit allows users to routinely verify the performance of their covaris. This instrument can used by customers for precisely shearing dna in preparation for illumina sequencing and other applications. Mechanical shearing for next generation sequencing ngs dnarna extraction from formalinfixed, paraffinembedded.